I-fluorescence microscope iwuhlobo lwesibonakhulu esibonakalayo esisebenzisa umthombo wokukhanya oqine kakhulu ukuze ukhanyise isifanekiso futhi ujabulise ama-fluorochrome kusampula. Ukukhanya kwesifanekiso kuvame ukwenziwa ngomthombo wokukhanya okhipha ukukhanya kwe-ultraviolet. Zisetshenziswa kakhulu emikhakheni yebhayoloji, yezokwelapha nezimboni.
Isebenza kanjani isibonakhulu se-fluorescence?
Isibonakhulu se-fluorescence sisebenzisa isibani se-mercury noma i-xenon ukukhiqiza ukukhanya kwe-ultraviolet. Ukukhanya kungena kusibonakhulu bese kushaya isibuko se-dichroic — isibuko esibonisa uhla olulodwa lwamaza amaza futhi sivumele obunye ububanzi ukuthi budlule. Isibuko se-dichroic sibonisa ukukhanya kwe-ultraviolet kuze kufike ku-specimen. Ezinye izibonelo zi-fluoresce ngokwemvelo ngaphansi kokukhanya kwe-ultraviolet ngoba ziqukethe izinto ezikhanyayo ezifana ne-chlorophyll. Uma isifanekiso esizobukwa singabi ne-fluoresce ngokwemvelo, singangcoliswa odayi be-fluorescent ababizwa ngokuthi ama-fluorochrome.
Iqukethe ini isibonakhulu se-fluorescence?
1. Umthombo Wokukhanya
Imithombo yokukhanya evame kakhulu yi-mercury, i-xenon, nama-LED. I-Mercury ihlinzeka ngekhwalithi engcono kakhulu yokukhanya yesibonakhulu se-fluorescence. Ama-LED aqala ukuthandwa kakhulu ngenxa yokuthi ayabiza kakhulu kuneminye imithombo futhi asebenzisa amandla amancane.
| Isibani seMercury | I-Xenon Lamp | I-LED | |
| I-Wavelength Yokukhanya Okukhishiwe | 350-370 nm | 400nm ~ 450nm | 400-700 nm |
| Ukuphila konke(ihora) | 200-300 | 400-600 | 10000 |
| Inzuzo | 1. Ukukhanya Okunamandla Okuphezulu, Imithombo Yokukhanya Evame Kakhulu. 2. Ikhipha i-ultraviolet enamandla kanye ne-blue-violet ukuze ijabulise zonke izinhlobo ze-fluorophores. 3. Izithombe ezikhanyayo nezinemibala. | 1. Ukuqina kwe-spectral kuzinzile. 2. Ukuqina kwe-spectral okunamandla ku-infrared naphakathi kwe-infrared. | 1. Kulula ukufaka esikhundleni. 2. Asikho isidingo sokufudumala. 3. Ingavulwa futhi icishwe khona manjalo. 4. Lawula ukuqina komthombo wokukhanya. |
| Ububi | 1. Isikhathi Esifushane Sokuphila. 2. Isikhathi eside sokufudumala. | 1. Ibhokisi lamandla elikhethekile eliphansi le-DC liyadingeka ukuze kushiswe ngokweqile. 2. Kubiza Kakhulu kuneMercury Lamp | 1. Isibani se-UV sibuthakathaka kunesibani semercury. 2.Ubude beza beza bokukhanya budinga i-LED ehlukile ngenxa yomkhawulokudonsa omncane abawukhiphayo. |
I-Mercury Fluorescent Attachment
Okunamathiselwe kwe-Fluorescent ye-LED
2. Isihlungi Sokuvusa
Isihlungi esivusa amadlingozi sibalulekile ekusebenzeni kwesibonakhulu se-fluorescence. Idlulisa ukukhanya kwe-wavelength emfushane, udayi we-fluorescent ongamunca. Futhi, ivimba eminye imithombo yokukhanya okujabulisayo.
Isihlungi Senjabulo(B,G,U,V)
3. I-Dichroic Mirror
Isibuko se-dichroic siwuhlobo lwesihlungi se-optical esibonisa ukukhanya kumaza wamaza athile kuyilapho sidlulisela amanye. Isetshenziswa kuma-microscopes e-fluorescence ukuhlukanisa amaza amaza okuvusa amadlingozi nokukhishwa.
4. Isihlungi Sokuphuma
Isihlungi se-emission sidlula kuphela ubude begagasi obukhishwa i-fluorophore futhi sivimba konke ukukhanya okungafuneki ngaphandle kwale bhendi - ikakhulukazi amaza ajabulisayo.
5. Odayi be-Fluorescent
Odayi be-fluorescent bayinhlanganisela yezinto eziphilayo ezinendawo ye-fluorescence, abangakha ngayo isithombe se-fluorescent ngokukhipha ukukhanya okuluhlaza okugqama kakhulu okubonakalayo ngemva kokujatshuliswa ukukhanya kwe-ultraviolet okukhanyisa kakhulu. Odayi be-fluorescent abasetshenziswa kakhulu yilaba; I-DAPI (49,6-diamidino-2-phenylindole), i-acridine orange, i-auramine-rhodamine, i-Alexa Fluors, noma i-DyLight 488.
Zingaki izinhlobo zamabonakhulu e-fluorescence?
1. I-Upright Epifluorescence Microscope:
Iwuhlobo oluvame kakhulu lwesibonakhulu se-fluorescence. Ukuvuswa kwe-fluorophore nokutholwa kwe-fluorescence kwenziwa ngendlela efanayo yokukhanya (okungukuthi ngomgomo). Iningi lamamicroscopes e-fluorescence, ikakhulukazi lawo asetshenziswa kwisayensi yempilo, angawomklamo we-epifluorescence.
I-BS-2081F Research Fluorescent Biological Microscope
2. I-Confocal Fluorescence Microscope:
I-Confocal Fluorescence Microscope: Lolu hlobo lwesibonakhulu se-fluorescence luhlanganisa ukuskena kwe-laser nokukhanya kwe-fluorescent ukuze kukhiqizwe isithombe. Ingasetshenziswa ezinhlelweni eziningi ezahlukahlukene, njengokufunda amaseli nezicubu, ukuthola amaprotheni nezinye izinto ngaphakathi kwamaseli, nokulinganisa ukushuba kwezinto.
I-BS-2081F Research Fluorescent Biological Microscope
3. IMbonakhulu yeFluorescence Ehlanekezelwe
Lolu hlobo lomthombo wokukhanya we-microscope kanye ne-condenser zitholakala phezulu, zibheke phansi. I-engeli yokukhanya kufanele ibe ngamadigri angu-90 ngokuphathelene nobuso besampula esihlolwayo.
I-BS-2095F I-Fluorescent Eguquliwe Isibonakhulu
Yiziphi izinzuzo kanye nobubi bezibonakhulu ze-fluorescence?
Izinzuzo:
- Inika amandla ukubonwa kwamaseli ezintweni eziphilayo ngaphandle kokuzilimaza
- Inikeza izithombe zokucaca okuphezulu ngemibala enembile
- Ivumela ukufundwa kwezinqubo ezibukhoma kumaseli
- Kusetshenziselwe ukuhlonza izinhlobo ezahlukene zama-molecule ngaphakathi kwamaseli, njengamaprotheni noma ama-nucleic acid.
Ububi:
-Ivumela kuphela ukubonwa kwezakhiwo ezithile ngaphakathi kweseli elimakwe ngodayi we-fluorescent.
-I-photobleaching ngenxa ye-electron excitation phakathi nenqubo ye-fluorescence ingase ithinte ama-molecule asebenzayo odayi be-fluorescent. Ngenxa yalokho, odayi abasebenzayo bangase balahlekelwe yimpahla yabo yamakhemikhali ye-fluorescence emission intensity.
-Amaseli asengozini yokuba nomthelela we-phototoxic ngemva kokungcoliswa odayi be-fluorescent, njengoba ama-molecule e-fluorophore amunca ama-photon wamandla aphezulu kusukela ekukhanyeni kwe-wavelength efushane.
Yiziphi izinhlelo zokusebenza ze-fluorescence microscope?
I-Fluorescence microscopes isetshenziswa kabanzi emikhakheni eyahlukene yocwaningo nokusebenzisa okuhlanganisa i-biochemistry, i-cell biology, i-microbiology, i-immunology, kanye nemithi.
1. Emkhakheni wesayensi yezinto eziphilayo, isibonakhulu se-fluorescence sinika amandla ukuhlonza okunembile nokunemininingwane yezingxenye zamaselula kanye ne-submicroscopic yeselula nemisebenzi ngosizo lokulebula kodayi we-fluorescent.
2. Emkhakheni wezokwelapha, isibonakhulu se-fluorescence singasebenzisa ama-reagents e-fluorescent ukuthola ubukhona nokusabalalisa amagciwane namagciwane, noma ukusiza ekulebuleni okuhlosiwe kokuhlinzwa ukuze kube lula ukuhlinzwa.
3. Emkhakheni we-mineralogy, isibonakhulu se-fluorescence sivame ukusetshenziselwa ukutadisha izinto ezinezakhiwo ezizenzakalelayo ze-fluorescence, njenge-asphalt, i-petroleum, amalahle, i-graphene oxide namanye amaminerali.
4. Kusayensi yezinto zokwakha, isibonakhulu se-fluorescence singasetshenziswa embonini yendwangu noma embonini yephepha ukuze kuhlaziywe izinto ezisekelwe ku-fiber.
Isikhathi sokuthumela: Mar-14-2023